Citation
Hoke, Morgan K.; McCabe, Kimberly A.; Miller, Aaron A.; & McDade, Thomas W. (2018). Validation of Endotoxin-Core Antibodies in Dried Blood Spots as a Measure of Environmental Enteropathy and Intestinal Permeability. American Journal of Human Biology, 30(4), e23120.Abstract
OBJECTIVE: To validate a method for measuring endotoxin-core antibodies (EndoCAb) from dried blood spots (DBS)-drops of capillary whole blood collected and dried on filter paper-as an indicator of environmental enteropathy (EE) in infancy and early childhood.METHODS: A commercially available enzyme immunoassay kit was adapted for use with DBS, with optimized steps for sample elution. Technical validation included analysis of linearity/recovery, precision and reliability, lower limit of detection, and correspondence between matched plasma and DBS samples. Validation in a field-based setting was implemented with samples from Peruvian infants (n = 82; age = 2-33 months) collected at two time points six months apart.
RESULTS: A high correspondence between plasma and DBS levels of EndoCAb was observed (R(2) = 0.93, P < .001). The lower limit of detection was found to be 0.01 GMU/mL. Interassay coefficient of variation (CV) was 10.9% and 8.06% for low and high controls, respectively. Mean intra-assay CVs were 3.22% and 1.83%, respectively. In a sample of Peruvian infants, EndoCAb levels increased with age as expected (P < .001). Age explained nearly 34.6% of the variance in EndoCAb across the sample.
CONCLUSION: These findings demonstrate the validity and feasibility of measuring EndoCAb in remote field settings using minimally invasive DBS sampling.
URL
http://dx.doi.org/10.1002/ajhb.23120Reference Type
Journal ArticleYear Published
2018Journal Title
American Journal of Human BiologyAuthor(s)
Hoke, Morgan K.McCabe, Kimberly A.
Miller, Aaron A.
McDade, Thomas W.